PPAR Research / 2013 / Article / Fig 4

Research Article

Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose

Figure 4

UV spectra and HPLC analysis of TOFA-CoA and C75-CoA. Ultraviolet spectra of TOFyl-CoA and C75-CoA were obtained with a Cary 100 Scan UV-Visible Spectrophotometer (Varian, Inc., Palo Alto, CA, USA) as described in Methods. TOFyl-CoA and C75-CoA were purified by HPLC as previously described [15]. When the final purified TOFyl-CoA and C75-CoA were reapplied to the HPLC column, representative HPLC runs detected only single absorbance peaks at 260 nm for TOFyl-CoA and C75-CoA with retention times of 13 and 9 min, respectively.