PPAR Research / 2013 / Article / Fig 8

Research Article

Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose

Figure 8

TOFA, TOFyl-CoA, and C75-CoA binding to L-FABP as determined by quenching of intrinsic L-FABP tyrosine quenching. L-FABP tyrosine quenching by TOFA (panel a), TOFyl-CoA (panel b), and C75-CoA (panel c) was determined as described in Methods. Tyrosine fluorescence emission of L-FABP (100 nM) in 10 mM phosphate buffer (pH = 7.4) was monitored by scanning from 290 to 400 nm, with excitation wavelength 280 nm, before and after small increments of added binding ligand. The binding curve was constructed by plotting percentage of tyrosine fluorescence intensity remaining at 305 nm versus concentration of the ligand. Panel (a) and (b), mean ± SEM, .