Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose
Selective fatty acid synthesis inhibitors and their CoA thioesters alter L-FABP secondary structure determined by circular dichroism (CD). L-FABP (1 μM) was incubated in the absence or presence of 10 μM ligand for 10 min at 25°C. Circular dichroism (CD) spectra were obtained utilizing a JASCO J-815 CD spectrometer (JASCO Analytical Instruments, Easton, MD, USA). Each sample spectrum represented the average of ten scans, and each sample spectrum was baseline corrected. Secondary structure was determined using the CONTIN algorithm as supplied by the instrument manufacturer. Statistical significance of secondary structure differences was determined by one-way ANOVA with the Newman-Keuls posttest (). * for L-FABP + ligand versus rat L-FABP (no ligand); ** for rat L-FABP + ligand versus rat L-FABP (no ligand); *** for rat L-FABP + ligand versus rat L-FABP (no ligand).