PPAR Research / 2013 / Article / Tab 1

Research Article

Inhibitors of Fatty Acid Synthesis Induce PPARα-Regulated Fatty Acid β-Oxidative Genes: Synergistic Roles of L-FABP and Glucose

Table 1

Binding constants of fatty acids, TOFA, and C75 to L-FABP and SCP-2.

LIGANDBinding constants ( M)
L-FABPSCP2

Fluorescent fatty acids
 NBD stearate = 0.018 ± 0.001 
= 0.055 ± 0.002
0.101 ± 0.003

Endogenous fatty acid
 Stearic acid0.087 ± 0.006(a)ND

Fatty acid synthesis inhibitors
 TOFA0.066 ± 0.003(a)
0.057 ± 0.004(c)
0.227 ± 0.020(a)
 C755.59 ± 0.31(b)not bound(a)

was determined by displacing protein-bound NBD-stearic acid (a) or ANS (b) as in Methods. (c), was determined by L-FABP tyrosine quenching. ND: not determined. Values are the mean ± SEM, .