Combined Effects of PPARγ Agonists and Epidermal Growth Factor Receptor Inhibitors in Human Proximal Tubule Cells

Figure 6

High-glucose-induced collagen IV (CIV) expression. Pioglitazone and gefitinib reduced high-glucose-induced CIV expression. The combination of both, pioglitazone and gefitinib has a similar effect. HK2 cells were incubated for 48 h with 5 mM glucose media (control), high glucose (HG) ± Piog (10  μM) ± gefitinib (0.5  μM) and levels of CIV were determined by Western blotting. Representative images for CIV and actin bands are shown. Normalized results are expressed as mean ± SEM; 𝑛=3. *𝑃<0.05 versus control.