Effect of Chronic Valproic Acid Treatment on Hepatic Gene Expression Profile in Wfs1 Knockout Mouse
Figure 4
qRT-PCR analysis of Wfs1 expression in hepatic tissue of WT and Wfs1 mutant mice. qRT-PCR mRNA expression is represented as the mean of quadruplicate per sample against the endogenous reference gene Actb. (a) Chronic treatment with valproic acid (VPA) induces upregulation of Wfs1 expression (; ). Expression level of Wfs1 follows a gene-dose relationship with Wfs1 genotype (; ). All data are presented as means ± SEM () and were compared by two-way analysis of variance (ANOVA). (b) Relationship between the expression levels of Wfs1 and Ppard, linear regression across all data points (), and expression levels of Ppard and Wfs1 were measured using qRT-PCR, each point corresponds to one animal.
