Research Article

Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells

Figure 11

Western blot analysis of effect of γ-tocotrienol, rosiglitazone, and GW9662 given alone or in combination on levels of (a) cyclin and CDKs and (b) cyclin kinase inhibitors and phosphorylated retinoblastoma in +SA cells. +SA cells were plated at 06 cells/100 mm culture dish and treated with control or treatment media for 4-day incubation period. Afterwards, whole cell lysates were prepared from each treatment group for subsequent separation by polyacrylamide gel electrophoresis (50 μg/lane) followed by Western blot analysis. Scanning densitometric analysis was performed on all the blots done in triplicate and the integrated optical density of each bond was normalized with corresponding β-actin, as shown in bar graphs below their respective Western blot images. Vertical bars in the graphs indicate the normalized integrated optical density of bands visualized in each lane ± SEM (arbitrary unit). * as compared with vehicle-treated controls.