Research Article

Synergistic Antiproliferative Effects of Combined γ-Tocotrienol and PPARγ Antagonist Treatment Are Mediated through PPARγ-Independent Mechanisms in Breast Cancer Cells

Figure 4

Western blot analysis to determine expression of PPARγ in mammary cancer cells. (a) PPARγ levels were determined in untreated +SA, MCF-7, and MDA-MB-231 cells and (b) PPARγ levels were determined after treatment with γ-tocotrienol, rosiglitazone, and GW9662 alone or in combination in +SA cells. +SA, MCF-7, and MDA-MB-231 cells were initially plated at 06 cells/100 mm culture dish and treated with control or treatment media for 4-day incubation period. Afterwards, whole cell lysates were prepared from each treatment group for subsequent separation by polyacrylamide gel electrophoresis (50 μg/lane) followed by Western blot analysis. Scanning densitometric analysis was performed on all the blots done in triplicate and the integrated optical density of each bond was normalized with corresponding β-actin, as shown in bar graphs below their respective Western blot images. Vertical bars in the graphs indicate the normalized integrated optical density of bands visualized in each lane ± SEM (arbitrary unit).