TRPV1 Activation Attenuates High-Salt Diet-Induced Cardiac Hypertrophy and Fibrosis through PPAR-δ Upregulation
Characterization of TRPV1 in myocardium and H9c2 cells. (a) TRPV1 protein level was detected in myocardium and H9c2 cells. (b) TRPV1 localization in H9c2 cells and cardiac tissues of WT mice was shown with immunofluorescence. TRPV1 was stained in green and the nucleus was stained using DAPI (blue color). Images are representative of three separate experiments. The scale bar denotes 50 μm. (c) Fura-2-loaded H9c2 cells were stimulated by increasing the concentration of capsaicin (0.1–10 μmol/L) in the presence or absence of TRPV1 antagonist iRTX; values were expressed as the mean ± SEM from six separate experiments. Cap: capsaicin. versus Cap (10 μmol/L) and ## versus Cap (1 μmol/L).