PPARα: A Master Regulator of Bilirubin Homeostasis

Figure 3

The high affinity PPARα agonist Wy14,643 increases HO-1, UGT1A1, and MRP2 mRNA levels in human vascular and hepatic cell models. Human umbilical vein endothelial cells (HUVEC) (a and b), coronary artery smooth muscle cells (CASMC) (c and d), human hepatocytes (HH; donor 1) (e and f), and HepG2 cells (g and h) were treated with vehicle (DMSO, 0.1%, v/v) or Wy14,643 (75 μM) for 24 H. HO-1, BVR, UGT1A1, and MRP2 mRNA levels were quantified from total RNA through quantitative RT-PCR analyses and normalized with the housekeeping RNA 28S. Values (mean ± SD) are expressed relatively to control (vehicle) set at 1. Statistically significant differences between vehicle- and Wy14,643-treated cells are indicated by asterisks (Student’s 𝑡-test: ^∗𝑃<0.05; ^∗∗∗𝑃<0.001; n.s: nonsignificant).

(a) HO-1—HUVEC

(b) BVR—HUVEC

(c) HO-1—CASMC

(d) BVR—CASMC

(e) UGT1A1—HH

(f) MRP2—HH

(g) UGT1A1—HepG2

(h) MRP2—HepG2