Huaier Cream Protects against Adriamycin-Induced Nephropathy by Restoring Mitochondrial Function via PGC-1 Upregulation

Figure 2

Huaier blocked ADR-induced mitochondrial dysfunction in vitro. (a) Mitochondrial morphology imaged by transmission electron microscopy. (b) Representative images of podocytes stained with dichlorodihydrofluorescein diacetate (DCFDA). (c) Quantitation of 2′,7′-dichlorofluorescein (DCF) fluorescence by flow cytometry. (d) Quantitation of MitoSOX fluorescence by flow cytometry. Podocytes were pretreated with Huaier (0.2 mg/mL) for 1 h followed by incubation with ADR (200 nM) for further 24 h. Data are expressed as the means ± SD (). versus control. versus ADR group.

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