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Figure 3: Huaier blocked ADR-induced mitochondrial dysfunction in vitro. (a) Representative images of podocytes stained with JC-1. (b) MMP measurements by flow cytometry. (c) Real-time RT-PCR analysis of mtDNA copy number. (d) ATP content. Podocytes were pretreated with Huaier (0.2 mg/mL) for 1 h followed by incubation with ADR (200 nM) for further 24 h. Data are expressed as the means ± SD (). versus control. versus ADR group.