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PPAR Research
Volume 2016, Article ID 2756781, 9 pages
Research Article

Peroxisome Proliferator-Activated Receptor γ Induces the Expression of Tissue Factor Pathway Inhibitor-1 (TFPI-1) in Human Macrophages

1Inserm, CHU Lille, Institut Pasteur de Lille, U1011, EGID, Université de Lille, 59000 Lille, France
2CHU, CNRS, Inserm, IRCAN, Université Côte d’Azur, Nice, France
3Department of Cardiology, RWTH Aachen University, Aachen, Germany

Received 14 September 2016; Accepted 28 November 2016

Academic Editor: Nanping Wang

Copyright © 2016 G. Chinetti-Gbaguidi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental Table 1. Baseline parameters of patients. Data are mean ± SD, n or median (interquartile range).

Supplemental Figure 1. PPARα and PPRβ/δ activation induces the expression of TFPI-1 in human primary macrophages. Expression of TFPI-1 was measured by Q-PCR in differentiated macrophages treated in the absence or in the presence of GW1516 (100 nM), GW647 (600 nM) or GW1929 (600 nM), for 24 h. Results are representative of those obtained from 3 independent macrophage preparations and are expressed relative to the levels in untreated cells set as 1. Each bar is the mean value ± SD of triplicate determinations. Statistically significant differences between treatments and controls are indicated (*p < 0.05; **p < 0.01).

Supplemental Figure 2. PPARγ activation does not modify TFPI-1 activity in human primary macrophages. TFPI-1 specific activity was measured in differentiated macrophages treated or not with GW1929 (600 nM) for 24 h.

Supplemental Figure 3. PPARγ activation reduces the TF/TFPI-1 ratio. Differentiated macrophages were treated with GW1929 (24 h, 600 nM), washed and then incubated in the presence of FVIIa (10 nM) for a further 24 h. TF and TFPI-1 mRNA levels were measured by Q-PCR and normalized to those of cyclophilin, and their ratio calculated and expressed as the mean value ± SD of triplicate determinations. Statistically significant differences are indicated (*p < 0.05).

  1. Supplementary Material