IL-15 Mediates Mitochondrial Activity through a PPARδ-Dependent-PPARα-Independent Mechanism in Skeletal Muscle Cells

Figure 7

Involvement of PPARα and PPARδ in IL-15 mediated mitochondrial activity in live cells. (a) Quantifiable fluorescence corrected for myotube size. Assessments were carried out on differentiated C2C12 myotubes following treatment with vehicle control (DMSO), IL-15 (100 ng/mL), IL-15 + GW-6471 (I + GW), IL-15 + GSK-3787 (I + GSK), and IL-15 + GW + GSK (I + G + G) every other day for 6 days during the differentiation protocol. (b) Representative images of mitochondrial activity assessment in live cells using a fluorescent probe sequestered into active mitochondria; Image J was used to quantify cell fluorescence. All values are displayed as means ± SEM, per group, from all groups; .

(a)

(b)