Puerarin Protects against Cardiac Fibrosis Associated with the Inhibition of TGF-β1/Smad2-Mediated Endothelial-to-Mesenchymal Transition

Figure 2

Puerarin protected against cardiac fibrosis by inhibiting EndMT. (a) Immunofluorescence colocalization of CD31 (red) and vimentin (green) was tested on frozen sections of mice hearts in indicated groups (scale bars: 200 μm). The left panel was positive and negative controls. Positive control: CD31 (red) counterstained with DAPI (blue) in the muscle of mouse; vimentin (green) counterstained with DAPI (blue) in the testis of mouse. Negative control: heart tissue incubated with PBS instead of primary antibodies against CD31 or vimentin. (b) Protein expressions of CD31 and vimentin were determined by WB, normalized to GAPDH . versus sham + vehicle group; versus TAC + vehicle group.

(a)

(b)