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PPAR Research
Volume 2017 (2017), Article ID 6139107, 7 pages
https://doi.org/10.1155/2017/6139107
Research Article

New Transcriptional Reporters to Quantify and Monitor PPARγ Activity

1INSERM, UMR-S1139, Faculté de Pharmacie de Paris, 75006 Paris, France
2Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France
3Fondation PremUp, 75006 Paris, France

Correspondence should be addressed to Séverine A. Degrelle; rf.oohay@enireves_ellerged

Received 10 July 2017; Revised 26 September 2017; Accepted 28 September 2017; Published 1 November 2017

Academic Editor: Constantinos Giaginis

Copyright © 2017 Séverine A. Degrelle et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The peroxisome-proliferator-activated-receptor-γ (PPARγ) is a member of the nuclear receptor superfamily that plays a critical role in diverse biological processes, including adipogenesis, lipid metabolism, and placental development. To study the activity of PPARγ, we constructed two new reporter genes: a fluorescent GFP-tagged histone-2B (PPRE-H2B-eGFP) and a secreted nanoluciferase (PPRE-pNL1.3[secNluc]). This study demonstrates their usage to monitor PPARγ activity in different cell types and screen for PPARγ’s potential ligands.