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PPAR Research
Volume 2017, Article ID 6139107, 7 pages
https://doi.org/10.1155/2017/6139107
Research Article

New Transcriptional Reporters to Quantify and Monitor PPARγ Activity

1INSERM, UMR-S1139, Faculté de Pharmacie de Paris, 75006 Paris, France
2Université Paris Descartes, Sorbonne Paris Cité, 75006 Paris, France
3Fondation PremUp, 75006 Paris, France

Correspondence should be addressed to Séverine A. Degrelle; rf.oohay@enireves_ellerged

Received 10 July 2017; Revised 26 September 2017; Accepted 28 September 2017; Published 1 November 2017

Academic Editor: Constantinos Giaginis

Copyright © 2017 Séverine A. Degrelle et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Figure S1: PPARγ: expression and role during trophoblast differentiation (fusion, hCG secretion). A) PPARγ in villous cytotrophoblast (VCT) and syncytiotrophoblast (ST). To the left- merged immunostaining in VCT and ST for PPARγ expression using PPARγ antibody (green), cell shape using F-actin (red) and nuclei using DAPI (blue). To the right- Immunostaining showing the differential expression of PPARγ between VCT and ST. PPARγ is highly expressed in ST. B) Fusion index of 48h-treated VCT with 1µM GW1929 (agonist of PPARγ) or 1µM GW9662 (PPARγ antagonist) compared to control (vehicle). C) hCG secretion of 48h-treated VCT with 1µM GW1929 (agonist of PPARγ) or 1µM GW9662 (PPARγ antagonist) compared to control (vehicle). Values are represented as mean ±S.D; ***P < 0.001, ****P < 0.0001 versus vehicle control (n=5).

Figure S2: PPRE-H2B-eGFP works in different cell types and species. Left panels- PPARγ is expressed in human primary cells (VCT/ST), BEWO, and mesenchyme, as well as in bovine extra-embryonic mesoderm cells. Displayed are the merged immunostaining for PPARγ expression using PPARγ antibody (green), cell shape using F-actin (red) and nuclei using DAPI (blue). Middle panels- only PPARγ expression (green). Left panels- PPARγ activity, PPRE-H2B-eGFP (green) and nuclei (Hoechst, blue).

  1. Supplementary Material