Inhibitory Effects of a Novel PPAR-γ Agonist MEKT1 on Pomc Expression/ACTH Secretion in AtT20 Cells

Figure 8

Effects of MEKT1 (time dependently), rosiglitazone, and pioglitazone on Nurr1, Nur77, and Tpit protein expression. (a) AtT20 cells treated with MEKT1 (M) at 10 μM for 24 hours, 6 hours, and 3 hours, rosiglitazone (R) at 10 μM for 24 hours, and pioglitazone (P) at 10 μM for 24 hours, or 0.1% DMSO as control (C) for 24 hours. Optical density (OD) of Nurr1/Nur77 was shown in figure (b), Nur77 in figure (c), and TBX19 (Tpit) in figure (d). OD of Nurr1/Nur77, Nur77, and TBX19 (Tpit) were normalized by OD of actin. Results are expressed as percentages of control (100%).

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