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Figure 2: Effect of Portulaca extract on serum cytokines and tissue injury scores of DSS-induced acute colitis. Panel (a) indicates that the mRNA expressions of TNF-α, IL-6, and IL-1β were evaluated in each group with qRT- PCR. Panel (b) shows that the levels of TNF-α, IL-6, and IL-1β in serum were detected via ELISA assay. Panel (c) displays the H&E stained colon images (NC refers to negative control; DSS refers to DSS alone group; M refers to mesalamine group; P refers to Portulaca group). The dotted potion indicated the hyperplasia of lymphoid follicle; red arrow pointed the infiltration of inflammatory cells. Panel (d) indicates the histological scores of H&E stained colorectum images. (Data were presented as mean ± SD from six rats. indicates comparison between DSS and NC; # indicates comparison between mesalamine and DSS; + indicates the comparison between Portulaca and DSS. The number of symbols indicates significance of difference; for example, four symbols indicate ; three symbols indicate ; two symbols indicate ; and one symbol indicates .)