Adenosine Receptor A1-A2a Heteromers Regulate EAAT2 Expression and Glutamate Uptake via YY1-Induced Repression of PPARγ Transcription

Figure 1

OGD promoted the interaction of A1AR with A2aAR in astrocytes. (a) Normal primary astrocyte morphology was observed by phase-contrast microscopy and is shown in the upper (×100) and lower (×200) panels. (b) Astrocytes were identified by immunofluorescence assay. The positive expression of the S100b (red) and GRAFP (green) antigens in astrocytes was determined by immunofluorescence (×200). Nuclei were stained with DAPI (blue). Astrocytes were exposed to OGD conditions for the indicated times. (c) The protein expression of A1AR, A2aAR, and EAAT2 was evaluated by western blotting. (d) Co-IP assays were used to evaluate the interaction between A1AR and A2aAR. (e) The intracellular glutamate levels were detected using a glutamate assay kit. The data represent the . . vs. the 0 h group and ^{^} vs. the 1 h group. The data represent as the ().

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