Adenosine Receptor A1-A2a Heteromers Regulate EAAT2 Expression and Glutamate Uptake via YY1-Induced Repression of PPARγ Transcription

Figure 4

The A1AR/A2aAR heterodimer regulated EAAT2 expression via YY1. (a) The YY1 protein level in astrocytes under OGD conditions for 1 h and 6 h was evaluated. (b) Western blot analysis of YY1 expression in cells treated with 30 nM CCPA and 200 nM SCH58261 that were exposed to OGD for 6 h. (c) The YY1 protein level in astrocytes transfected with A2aAr siRNA for 48 h, followed by exposure to OGD conditions with 30 nM CCPA, was evaluated. The expression of PPARγ and YY1 in treated astrocytes was evaluated by western blotting. (d) The YY1 protein after the transfection of YY1 siRNA into astrocytes for 48 h was evaluated by western blotting. YY1-silenced astrocytes were subjected to OGD conditions in the absence or presence of 30 nM CCPA. (e, f) The expression of YY1 and EAAT2 and the intracellular glutamate level were detected by western blotting or with a glutamate assay kit, respectively. The data represent the (). vs. the NC group and ^{^}vs. the OGD/NC group.

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