Loss of Hepatocyte-Specific PPARγ Expression Ameliorates Early Events of Steatohepatitis in Mice Fed the Methionine and Choline-Deficient Diet

Figure 3

MCD diet reduced hepatic fatty acid uptake but increased fatty acid uptake in adipose tissue. Plasma BODIPY-C16 levels in mice fed (a) MSD diet or (b) MCD diet. (c) Area under the curve of plasma BODIPY-C16 levels. (d) Liver-, (e) heart-, (f) urogenital (UG) fat-, (g) subcutaneous (SC) fat-, and (h) brown adipose tissue- (BAT-) specific uptake of BODIPY-C16. RFU: relative fluorescence units. Values of RFU/tissue are represented as the percentage of control mice fed a MSD diet (e–h). Letters represent significant differences between MSD and MCD within groups. ^A; ^B; ^C; ^D. Control mice (C); Pparg^ΔHep mice (KO); Pparg^ΔHep mice with hepatocyte Cd36 overexpression (KO+Cd36). MSD diet: open columns, open symbols; MCD diet: close columns, close symbols. In (a–c), control mice (open columns, discontinuous lines), KO mice (grey lines and columns), and KO+Cd36 mice (black lines and columns). mice/group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)