Apigenin Alleviates Liver Fibrosis by Inhibiting Hepatic Stellate Cell Activation and Autophagy via TGF-β1/Smad3 and p38/PPARα Pathways

Table 2

Oligonucleotide sequences of primers used for qRT-PCR.

Gene name	Forward (5-3)	Reverse (5-3)
β-Actin	GTGACGTTGACATCCGTAAAGA	GCCGGACTCATCGTACTCC
IL-1β	GAAATGCCACCTTTTGACAGTG	TGGATGCTCTCATCAGGACAG
Collagen 1	CAATGGCACGGCTGTGTGCG	AGCACTCGCCCTCCCGTCTT
α-SMA	CCCAGACATCAGGGAGTAATGG	TCTATCGGATACTTCAGCGTCA
MMP2	GGACAAGTGGTCCGCGTAAA	CCGACCGTTGAACAGGAAGG
TIMP1	CGAGACCACCTTATACCAGCG	ATGACTGGGGTGTAGGCGTA
p62	GAGGCACCCCGAAACATGG	ACTTATAGCGAGTTCCCACCA
LC3	TTATAGAGCGATACAAGGGGGAG	CGCCGTCTGATTATCTTGATGAG
Beclin-1	ATGGAGGGGTCTAAGGCGTC	TGGGCTGTGGTAAGTAATGGA
TGF-β1	CCACCTGCAAGACCATCGAC	CTGGCGAGCCTTAGTTTGGAC
PPARα	AACATCGAGTGTCGAATATGTGG	CCGAATAGTTCGCCGAAAGAA

Abbreviation: qRT-PCR: quantitative real-time PCR.