Research Article
PPAR-γ Agonist Pioglitazone Restored Mouse Liver mRNA Expression of Clock Genes and Inflammation-Related Genes Disrupted by Reversed Feeding
Table 3
Histopathological and morphometrical data of liver tissue of experimental animals.
| | Histological checklist | Time of feeding | | Daytime | Nighttime | | Untreated mice () | PG at 7 AM () | PG at 7 PM () | Control mice () | PG at 7 AM () | PG at 7 PM () |
| | Hepatocellular cords and/or sinusoid destruction, cases per group | | | | | | | | Diameter of hepatocyte nuclei in zone 2, μm, | | | | | | | | Nonparenchymal cells count per 1000 μm2, | | | | | | | | Liver cell injury: ballooning, cases per group | 8 (66.7%) | 6 (75.0%) | 10 (100.0%) | 6 (60.0%) | 8 (88.9%) | 7 (70.0%) | | Liver cell injury: acidophil bodies, cases per group | 7 (58.3%) | 4 (50.0%) | 2 (20.0%) | 0 (0.0%) | 2 (22.2%) | 0 (0.0%) | | Liver cell injury: pigmented macrophages, cases per group | 0 (0.0%) | 1 (12.5%) | 0 (0.0%) | 0 (0.0%) | 0 (0.0%) | 0 (0.0%) | | Lobular inflammation, cases per group | 5 (41.7%) | 5 (62.5%) | 3 (30.0%) | 4 (40.0%) | 4 (44.4%) | 8 (80.0%) | | Portal inflammation, cases per group | 0 (0.0%) | 1 (12.5%) | 0 (0.0%) | 1 (10.0%) | 0 (0.0%) | 1 (10.0%) | | Circulation alteration: cases of sludge per group | 8 (66.7%) | 4 (50.0%) | 6 (60.0%) | 3 (30.0%) | 2 (22.2%) | 4 (40.0%) | | Circulation alteration: cases of stasis per group | 11 (91.7%) | 8 (100.0%) | 10 (100.0%) | 9 (90.0%) | 8 (88.9%) | 9 (90.0%) | | Circulation alteration: cases of plasmostasis per group | 7 (58.3%) | 5 (62.5%) | 7 (70.0%) | 4 (40.0%) | 4 (44.4%) | 5 (50.0%) |
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value calculated by one-way ANOVA and post-hoc Bonferroni tests and Fisher’s exact test statistically significant differences ( ). |
