Research Article

Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark®) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens

Figure 2

HERmark Assay Workflow. Deparaffin/rehydration, antigen retrieval, and overnight antibody incubation steps are performed on Day 1. On day 2, the tumor tissue is incubated with the photosensitizer molecule and illuminated to release VeraTags. VeraTags are collected and separated on capillary electrophoresis and the tumors are H & E stained, tumor area identified and circled and the final sample and batch normalized data is typically available within a 7 day turnaround time.
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