Research Article

Effects of the Demethylating Agent, 5-Azacytidine, on Expression of the Kallikrein-Kinin Genes in Carcinoma Cells of the Lung and Pleura

Figure 1

Basal kallikrein-kinin mRNA and protein expression in H2126 lung adenocarcinoma, H520 lung squamous cell carcinoma, and nonmalignant BEAS-2B lung epithelial cells. (a) Fold differences in mRNA expression for the tissue kallikrein (KLK1), plasma kallikrein (KLKB1), kinin B1 receptor (BDKRB1), kinin B2 receptor (BDKRB2), and high molecular weight kininogen (KNG1) genes in H2126 cells (white bars) and H520 cells (black bars) relative to that in BEAS-2B cells. (b) Mean cytoplasmic expression of the kallikrein-kinin proteins in BEAS-2B cells (white bars), H2126 cells (hatched bars), and H520 cells (black bars), as assessed by immunofluorescence labelling and image analysis.