Morphological Analysis of CDC2 and Glycogen Synthase Kinase 3β Phosphorylation as Markers of G2 → M Transition in Glioma
Figure 2
pThr161CDC2 levels in glial tumors. Tissue arrays for GBM (a–c), oligodendroglioma (d–f), and ependymoma (g–i) were stained with anti-pThr161CDC2 antibodies. Groups were separated into low labeling (a, d, and g), intermediate labeling (b, e, and h), and high labeling (c, f, i) and representative photographs are illustrated. Bottom left of each panel shows the total number of cores () and their proportion of total cores for the low, intermediate, and high labeling group. Arrowheads show nuclear pThr161CDC2 labeling. Arrows show cytoplasmic pThr161CDC2 labeling in mitotic cells. Double arrows show pThr161CDC2 in non-mitotic cells. A greater proportion of cores from the GBM samples were in the high labeling group. (GBM = glioblastoma; Oligo = oligodendroglioma; EP = ependymoma; scale bar in yellow represents 100 μm).
