Morphological Analysis of CDC2 and Glycogen Synthase Kinase 3β Phosphorylation as Markers of G2 → M Transition in Glioma
Figure 4
High-grade gliomas show increased phospho-CDC2 levels relative to low-grade gliomas. (a) shows a mitotic figure from a GBM showing strong labeling for pTyr15CDC2; in (b), a mitotic figure from an anaplastic oligodendroglioma (WHO grade III) is shown with strong pTyr15CDC2 labeling. (c) shows the average number of positive cells/3 high power fields of the GBM, oligodendroglioma (Oligo), and ependymoma (EP) arrays (error bars show standard deviation). (d) shows the intracase correlation between pTyr15CDC2 and pThr161CDC2. The -axis of (d) is the percentage of total cores (i.e., denominator = no. of cores examined for each tumor type). The black bar denotes the percentage of cores where there was both low pTyr15CDC2 and low pThr161CDC2 labeling. The grey bar denotes percentage of cores where there is low pTyr15CDC2 with high pThr161CDC2 labeling. The blue bar denotes percentage of cores where there is high pTyr15CDC2 with low pThr161CDC2 labeling. The red bar denotes cores where there is high pTyr15CDC2 with high pThr161CDC2. Half of the GBM cores showed high pTyr15CDC2 with high pThr161CDC2 labeling, whereas the most common pattern seen in the oligodendroglioma and ependymoma cores was low pTyr15CDC2 with low pThr161CDC2. (e) shows the results of pTyr15CDC2 and pThr161CDC2 labeling observed in oligodendroglioma grade II versus grade III. Oligodendroglioma WHO grade III showed more cores with elevated CDC2 labelling. (pTyr = pTyr15CDC2; pThr = pThr161CDC2; L = low labeling; H = high labeling; ND = none detected).
