An Attempt to Establish a Common Animal Model for Hepatorenal Fibrosis in Rats

Figure 2

Comparison of histopathology among the groups. (a) Liver histopathology shows marked liver fibrosis in the model group and CCl₄ group. HE stain in the Model group shows periportal fibrosis and fibrous septa formation. Anti--SMA antibody positive staining is observed in the fibrotic areas and periportal/perivascular areas. Picro-Sirius red staining corresponds to the changes seen in HE stain. Histopathology of CCl₄ group also shows similar changes as that of Model group along with fatty changes. BSA group and Normal group show no changes except for edema and mild connective tissue proliferation in the BSA group. (b) Histopathology of kidney from Model group and BSA group shows focal areas of connective tissue proliferation and inflammatory cell infiltrates. Anti--SMA antibody positive staining is observed in the fibrotic regions and perivascular regions of Model, CCl₄, and BSA group. Picro-Sirius red staining shows similar intensity of collagen staining between Model, CCl₄, and BSA group.

(a)

(b)