Research Article

The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines

Figure 3

Changes in mRNA levels of cyclooxygenase-2 (COX-2) and representative western blots of prostaglandin E2 (PGE2) expression after LED irradiation. COX-2 mRNA is shown in (a), and representative western blots of PGE2 expression after LED irradiation are shown in (b, c, and d). Compared with the C groups, significant upregulation of COX-2 and PGE2 was noted in the I groups. Significantly decreased expression of COX-2 and PGE2 was noted in the LED irradiation groups compared with the incision only group. C indicates LED irradiation for 6 days on sham skin incision in the paw contralateral to the LED-irradiated paw; I-3 h, I-3 d, and I-6 d indicate incision only groups that underwent behavioral testing 3 hours, 3 days, and 6 days after skin incision; L-I indicates LED irradiation for 6 days before skin incision; I-L indicates LED irradiation for 6 days after skin incision; L-I-L indicates LED irradiation from 3 days before skin incision to 3 days after skin incision. compared with the I groups. The values represent the means ± SD.
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