The Antinociceptive Effect of Light-Emitting Diode Irradiation on Incised Wounds Is Correlated with Changes in Cyclooxygenase 2 Activity, Prostaglandin E2, and Proinflammatory Cytokines

Table 1

Real-time polymerase chain reaction primers.

Gene	Direction	Primers
COX-2	Fwd.	5′-TGT ATG CTA CCA TCT GGC TTC GG-3′
	Rev.	5′-GTT TGG AAC AGT CGC TCG TCA TC-3′
IL-6	Fwd.	5′-CAT ATG TTC TCA GGG AGA TCT TGG A-3′
	Rev.	5′-CAG TGC ATC ATC GCT GTT CAT AC-3′
IL-1β	Fwd.	5′-CAC AGC AGC ATC TCG ACA AGA G-3′
	Rev.	5′-GAC ATA GGT AGC TGC CAC AGC TT-3′
TNF-α	Fwd.	5′-AGG CTG CCC CGA CTA TGT-3′
	Rev.	5′-AGG AGG CTG ACT TTC TCC-3′
β-Actin	Fwd.	5′-CGT ACC ACT GGC ATT GTG ATG-3′
	Rev.	5′-CAC GCT CGG TCA GGA TCT TC-3′

The COX-2, IL-6, IL-1β, TNF-α, and β-actin primer sequences were derived from the National Center for Biotechnology Information (Bethesda, Virginia) nucleotide sequence (accession numbers NM_017232.3, NM_012589.2, NM_031512.2, NM_012675.3, and NM_031144.3, resp.).