Low-Concentration Oxygen/Ozone Treatment Attenuated Radiculitis and Mechanical Allodynia via PDE2A-cAMP/cGMP-NF-κB/p65 Signaling in Chronic Radiculitis Rats

Figure 4

20 μg/mL oxygen/ozone suppressed the expression of phosphodiesterase 2A (PDE2A) and nuclear factor- (NF-) κB/p65 mRNA and protein on day 7 in chronic radiculitis rats. The expression of both PDE2A mRNA (a) and protein (b) in the ipsilateral spinal cord was significantly increased on day 7 in the chronic radiculitis control group (). 20 μg/mL O₃ significantly reversed the overexpression of PDE2A that was induced by autologous nucleus pulposus implantation (). The expressions of both NF-κB/p65 mRNA (c) and protein (d) in the spinal cord were also elevated on day 7 in the control group (). Both NF-κB/p65 mRNA and protein expressions were significantly decreased in the 20 μg/mL O₃ group () compared to the control group. The values are expressed as mean ± SEM. vs. sham group; vs. control group.

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