Pain Research and Management

Pain Research and Management / 2019 / Article
Special Issue

Basic and Clinical Advances in the Diagnosis and Management of Migraine

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Research Article | Open Access

Volume 2019 |Article ID 7890461 | 6 pages | https://doi.org/10.1155/2019/7890461

Effects of Diet Based on IgG Elimination Combined with Probiotics on Migraine Plus Irritable Bowel Syndrome

Academic Editor: Aneta Wieczorek
Received17 Apr 2019
Revised17 Jul 2019
Accepted28 Jul 2019
Published21 Aug 2019

Abstract

Several research studies have revealed that migraine has a solid link with gastrointestinal diseases especially irritable bowel syndrome (IBS). This study was carried out to investigate therapeutic potential of diet based on IgG elimination combined with probiotics on migraine plus irritable bowel syndrome. A total of 60 patients diagnosed with migraine plus IBS were recruited for the study. IgG antibodies against 266 food varieties were detected by ELISA. Then, the subjects were randomized into three groups for treatment of IgG elimination diet or probiotics or diet combined with probiotics. Migraine symptom, gut function score, medication use, and serum serotonin level were measured at baseline, 7 weeks, and 14 weeks. Improvement of migraine and gut symptom was achieved at a certain time point. Reduced use of over-the-counter- (OTC−) analgesics was seen in all groups. However, use of triptans did not show significant difference. An increased serum serotonin level was seen in subjects treated with elimination diet and elimination diet combined with probiotics. IgG elimination diet combined with probiotics may be beneficial to migraine plus IBS. It may provide new insight by understanding the intricate relationship between migraine and gastrointestinal diseases.

1. Introduction

Migraine is described as a debilitated headache with a prevalence of 13–33% over a lifetime. Patients may suffer severely from the symptoms as well as a high economic burden [1]. However, the underlying mechanisms are still not fully understood. There is growing evidence indicating that central nervous system (CNS) manifestations may appear after the gastrointestinal dysfunction [2]. The interactive relationship between the intestine and the brain is termed as the “gut-brain axis” [3]. Gratifying achievements have been made in delineating the bidirectional relationship between the CNS and the intestinal tract. Emerging evidence suggests that migraine patients tend to get gastrointestinal diseases and patients with gastrointestinal (GI) diseases are more liable to catch migraine, as compared to healthy controls [46]. Among these patients, migraine concomitant IBS is most commonly seen [79]. Growing evidence indicates that the intestinal microbiota and its metabolites may manage GI functions by affecting intestinal sensitivity and motility, intestinal permeability, and mucosal immune function [10, 11]. Undigested food particles and bacterial metabolite may enter the bloodstream and affect intestinal function [12], the leaky gut hypothesis suggests that intestinal disorders may prompt increased intestinal permeability, and then bacterial by-products such as lipopolysaccharides may flow into the bloodstream and ultimately cause a response provoking migraine [13, 14]. Moreover, intestinal microbiotas have been found to have a solid impact on neurotransmitter levels, especially serotonin (5-HT) which plays a significant role in migraine [15, 16]. Thus, amending function of the intestine may ameliorate intensity and duration time of migraine attacks. Probiotics, as living microorganisms, have been verified to stabilize the intestinal epithelial barrier in multiple ways [17]. Reduced pathogenic bacteria have been found when administered in probiotic bacterial strains by secreting antimicrobial factors. Furthermore, increased mucus output of the goblet cells has been found and they are of great importance for the tight junctions between the intestinal epithelial cells [18]. Several researchers found that diet based on elimination of certain food could reduce the occurrence and severity of migraine attacks [19, 20]. Abundance of food-specific IgGs may indicate food hypersensitivity. Hence, consumption of IgG-free food could ameliorate clinical manifestation of migraine.

Herein, we explored effects of diet based on IgG elimination combined with probiotics on migraine plus IBS, adding to growing evidence that management of intestinal function may be beneficial for migraine patients.

2. Materials and Methods

2.1. Subjects and Ethics

This study was carried out at The First Affiliated Hospital of University of South China. Sixty patients were enrolled in the study from May 2017 through December 2018 in the internal medicine department. International Classification of Headache Disorders, 3rd edition (beta version) (ICHD-3-beta), was employed to diagnose migraine; all patients were accompanied with uncomplicated IBS (bowel habit subtypes) according to the Rome III criteria. Five subjects were excluded due to difficulty in keeping the diet.

For meeting the inclusion criteria, the patients should (I) be aged between 18 and 65 years, (II) be diagnosed with migraine for more than 6 months and have at least 4 headache days within the last month, (III) have discomfort in the gut for more than 12 weeks in the past year, and (IV) be treated with preventive medications or acute attack medications unchanged for more than 6 months. Patients who have a definite history of medication overuse, headache, menstrual or other associated headache disorder, and organic abdominal diseases were excluded from the experiment.

Informed consent was obtained from subjects, and all the procedures were approved by the Institutional Review Board of the University of South China.

2.2. IgG Antibody Detection against Food Antigens and Diet Preparation

IgG antibodies against 266 food antigens were measured by a commercially available enzyme-linked immunosorbent assay (ELISA) kit (ImuPro 300 test; Evomed/R-Biopharm AG, Darmstadt, Germany). Quantitative measurements were reported in mg/l. Values above 7.5 mg/l were considered as positive reaction to the corresponding food. These samples were graded according to their titres, “low” for titres between 7.5–12.5 mg/l; “moderate” for 12.51–20 mg/l; “high” for 20.1–50 mg/l; and “very high” for 50.1–200 mg/l. According to the IgG antibody results, the elimination diet was composed of IgG negative food and the normal diet was made up with IgG-negative and IgG-positive food. There was no difference in calorie contents between these two diets. Subjects were guided to follow the diet (IgG-negative or IgG-positive) arranged by dietician.

2.3. Experiment Procedures and Measurement

A double-blind, randomized, controlled cross-over clinical trial was performed, and participants were randomly assigned to three groups which include subjects with elimination diet, probiotics, or elimination diet combined with probiotics. The probiotics product contains the following bacterial strains (Bifidobacterium infantis, Lactobacillus acidophilus, Enterococcus faecalis, and Bacillus cereus), and the subjects consumed 1.5 grams three times a day for 14 weeks.

During the experiment period, the subjects were requested to fill out a headache questionnaire, the Migraine Disability Assessment Scale (MIDAS), to evaluate severity of the migraine. IB Severity Scale (IBSS) was applied to assess the therapeutic effects of the intestine. A spectrophotofluorimetric method was applied to measure the concentration of serotonin in plasma [21]. Each scale and concentration of 5-HT in serum were assessed every 7 weeks.

2.4. Statistical Analysis

All data are expressed as mean ± S.D. Experiments with three or more groups were compared by ANOVA, followed by the LSD test. was taken significant.

3. Results

3.1. Result of IgG Antibody Tests

Of the total 1506 reactions, 660 (43.8%) were graded as “low,” 693 (46%) were “moderate,” 105 (7%) were “high,” and 48 (3.2%) were “very high”. Food types are listed in Table 1.


Food typesNumber of patients with positive test result (n = 60)

Spices53
Seeds and nuts50
Grain with gluten48
Seafood43
Food additives26
Eggs26
Cheese24
Sugar products24
Milk product24
Grain without gluten19
Vegetable14
Coffee infusions10
Salads5
Yeast5
Meat5
Mushrooms3

3.2. Migraine Symptoms

As shown in Figure 1(a), migraine days of subjects with elimination diet in 14 weeks and elimination diet combined with probiotics in 7 and 14 weeks were significantly decreased. However, subjects with probiotics showed no difference. In 7 weeks, the mean MIDAS score decreased significantly only in subjects with elimination diet combined with probiotics. However, all groups exhibited an evident decrease in 14 weeks compared to baseline data (Figure 1(b)).

3.3. IBS Symptoms

As shown in Figure 2, in 14 weeks, a remarkable improvement was observed with all groups in bowel habit, compared to baseline data. No difference was found in 7 weeks. Only subjects with elimination diet combined with probiotics showed improvement in 14 weeks, referring to severity of abdominal distention.

3.4. Use of Medication

As shown in Figure 3, the use of triptans did not alter in all groups. The use of over-the-counter analgesics decreased in all groups in 14 weeks, only subjects with elimination diet combined with probiotics showed improvement in 7 weeks.

3.5. Concentration of 5-HT in Serum

As shown in Figure 4, in 14 weeks, subjects with elimination diet or elimination diet combined with probiotics exhibited a significant increase in concentration of 5-HT in serum compared to baseline data. No difference was found in other groups and time points.

4. Discussion

To our knowledge, it is the first research to prove that IgG elimination diet combined with probiotic may be beneficial to migraine plus IBS. Meanwhile, compliance was high and relevant adverse reactions did not happen. According to our findings, IgG-mediated food allergies have been proved to play an important role in migraine attacks although the mechanism is not fully illuminated. There is emerging proof that inflammation acts as a crucial role in the pathogenesis of migraine [22, 23]. A specific marker is needed if we focus on inflammation response prompted by certain foods. All IgG subclasses except IgG4 cause inflammatory response after contact with specific antigen [24]. Thus, identifying IgG for variety of foods may be applicable to detect allergized food and give guidance for amendment of dietary habits so as to keep away from chronic inflammation and onset of migraine. IBS patients were proved to have a greater gut permeability defect than healthy controls. Thus, increased intake of dietary antigens to lamina propria occurred in individuals with IBS. Decreased lymphocyte proliferation and release of inflammatory cytokines were found when consuming customized elimination diet [25]. Several studies have indicated that probiotics have therapeutic efficacy in gastrointestinal diseases [26, 27]. The potential mechanism of probiotics in treating gut-associated diseases may strengthen intestinal barrier function in several ways. Meanwhile, it may impact pain pathways by influencing brain signaling [28]. Hence, probiotics may relieve migraine headache by amending the intestinal barrier function through the gut-brain axis. Serotonin is neurogenic and serves a pivotal role in cell differentiation, division, and migration [29]. The enteric nervous systems are made up of more than 100 million neurons, and they may communicate with the central nervous system bidirectionally and continuously through several mediators [30]. Of these mediators, serotonin is mostly researched. Several studies suggest that serotonin is an important link in the brain-gut axis. However, only 3% of the whole serotonin of human is located in the central nervous system. The rest is located in the intestine. Enteric bacteria have been elucidated to regulate production of serotonin. Therefore, amending the function of intestine may be a way to cure migraine patients. The migraine days significantly decreased in the diet group in 7 and 14 weeks and got better with time. Also, the diet group exhibited significant change in 14 weeks. Although decrease was seen in the probiotic group, it showed no statistical significance. This may be explained as probiotics may take effect slower than other groups. Improvement was seen in the combined group in 14 weeks, referring to abdominal distention; however, other groups did not show a significant change because the treatment was inadequate. Reduction in triptan use was not seen in all groups, and this may be explained as some subjects were hard to get rid of it due to drug dependence.

Our data are in consistence with other studies which report IgG elimination diet or probiotics are beneficial for migraine plus IBS. Compared to these studies, ours has many superiorities. To begin with, we found that subjects with IgG elimination diet combined with probiotics had effect quicker than other groups when considering migraine symptom (MIDAS score and migraine days). Secondly, although all the groups did not reduce the use of triptans, use of over-the-counter analgesic decreased dramatically in all groups in 14 weeks and only diet combined with probiotics showed effect at 7 weeks, and this indicated it may show effects quicker than other groups in nonacute attack. Moreover, improvement in bowel habit was seen in all groups in 14 weeks, but only subjects with diet combined with probiotics exhibited a relief in severity of abdominal distention. Thus, diet combined with probiotics may be an optimal selection for the management of intestinal function. Finally, titres of serotonin were upregulated in 14 weeks in subjects with diet and diet combined with probiotics, but the latter showed a greater magnitude. However, we must pay attention when translating these results into daily practice due to limited sample size. Further study is required to elucidate the underlying mechanism of IgG-positive, food-induced migraine.

5. Conclusions

In summation, we provide the first clinical evidence that IgG elimination diet combined with probiotics may be beneficial to migraine plus IBS. Future work should uncover the potential mechanism of how it affects pathophysiology of migraine.

Data Availability

The original data are available from the corresponding author upon request.

Conflicts of Interest

The authors declare that there are no conflicts of interest.

Acknowledgments

This study was supported by grants from the Natural Science Foundation of Hunan Province (Grant No. 2017JJ3273) and the Hengyang Science and Technology Development Foundation (Grant No. 2016KJ38).

References

  1. U. Uygunoglu and A. Siva, “Epidemiology of headache,” Cephalalgia An International Journal of Headache, vol. 21, pp. 774–777, 2010. View at: Google Scholar
  2. M. Rao and M. D. Gershon, “The bowel and beyond: the enteric nervous system in neurological disorders,” Nature Reviews Gastroenterology & Hepatology, vol. 13, no. 9, pp. 517–528, 2016. View at: Publisher Site | Google Scholar
  3. E. A. Mayer, K. Tillisch, and A. Gupta, “Gut/brain axis and the microbiota,” Journal of Clinical Investigation, vol. 125, no. 3, pp. 926–938, 2015. View at: Publisher Site | Google Scholar
  4. X. Moisset, G. Bommelaer, M. Boube et al., “Migraine prevalence in inflammatory bowel disease patients: a tertiary-care centre cross-sectional study,” European Journal of Pain, vol. 21, no. 9, 2017. View at: Publisher Site | Google Scholar
  5. N. Hindiyeh and S. K. Aurora, “What the gut can teach us about migraine,” Current Pain & Headache Reports, vol. 19, no. 7, pp. 1–8, 2015. View at: Publisher Site | Google Scholar
  6. H. Rahmoune and N. Boutrid, “Migraine, celiac disease and intestinal microbiota,” Pediatric Neurology Briefs, vol. 31, no. 2, p. 6, 2017. View at: Publisher Site | Google Scholar
  7. C.-I. Lau, C.-C. Lin, W.-H. Chen, H.-C. Wang, and C.-H. Kao, “Association between migraine and irritable bowel syndrome: a population-based retrospective cohort study,” European Journal of Neurology, vol. 21, no. 9, pp. 1198–1204, 2014. View at: Publisher Site | Google Scholar
  8. D. Georgescu, D. Reisz, C. V. Gurban et al., “Migraine in young females with irritable bowel syndrome: still a challenge,” Neuropsychiatric Disease & Treatment, vol. 14, pp. 21–28, 2018. View at: Publisher Site | Google Scholar
  9. E. I. Aydinlar, P. Y. Dikmen, A. Tiftikci et al., “IgG-based elimination diet in migraine plus irritable bowel syndrome,” Headache: The Journal of Head and Face Pain, vol. 53, no. 3, pp. 514–525, 2013. View at: Publisher Site | Google Scholar
  10. B. Hippe, M. Remely, N. Bartosiewicz et al., “Abundance and diversity of GI microbiota rather than IgG4 levels correlate with abdominal inconvenience and gut permeability in consumers claiming food intolerances,” Endocrine‚ Metabolic & Immune Disorders-Drug Targets, vol. 14, no. 1, pp. 67–75, 2014. View at: Publisher Site | Google Scholar
  11. M. L. Robinette and M. Colonna, “GI motility: microbiota and macrophages join forces,” Cell, vol. 158, no. 2, pp. 239-240, 2014. View at: Publisher Site | Google Scholar
  12. R. Mennigen and M. Bruewer, “Effect of probiotics on intestinal barrier function,” Annals of the New York Academy of Sciences, vol. 1165, no. 1, pp. 183–189, 2010. View at: Publisher Site | Google Scholar
  13. M. Maes, “The cytokine hypothesis of depression: inflammation, oxidative & nitrosative stress (IO&NS) and leaky gut as new targets for adjunctive treatments in depression,” Biogenic Amines, vol. 29, no. 3, pp. 186–194, 2008. View at: Google Scholar
  14. E. M. Quigley, “Leaky gut—concept or clinical entity?” Current Opinion in Gastroenterology, vol. 32, no. 2, pp. 74–79, 2016. View at: Publisher Site | Google Scholar
  15. S. M. O’Mahony, G. Clarke, Y. E. Borre, T. G. Dinan, and J. F. Cryan, “Serotonin, tryptophan metabolism and the brain-gut-microbiome axis,” Behavioural Brain Research, vol. 277, pp. 32–48, 2014. View at: Google Scholar
  16. T. Jenkins, J. Nguyen, K. Polglaze, and P. Bertrand, “Influence of tryptophan and serotonin on mood and cognition with a possible role of the gut-brain axis,” Nutrients, vol. 8, no. 1, p. 56, 2016. View at: Publisher Site | Google Scholar
  17. S. Hempel, S. J. Newberry, A. R. Maher et al., “Probiotics for the prevention and treatment of antibiotic-associated diarrhea: a systematic review and meta-analysis,” JAMA the Journal of the American Medical Association, vol. 307, no. 18, pp. 1959–1969, 2012. View at: Publisher Site | Google Scholar
  18. Y. J. Dai, H. Y. Wang, X. J. Wang, A. D. Kaye, and Y. H. Sun, “Potential beneficial effects of probiotics on human migraine headache: a literature review,” Pain Physician, vol. 20, no. 2, pp. E251–E255, 2017. View at: Google Scholar
  19. W. Feldman, J. Egger, J. Wilson, C. M. Carter, M. W. Turner, and J. F. Soothill, “Is migraine food allergy?” The Lancet, vol. 322, no. 8364, p. 1424, 1983. View at: Publisher Site | Google Scholar
  20. K. Alpay, M. Ertaş, E. K. Orhan, D. K. Üstay, C. Lieners, and B. Baykan, “Diet restriction in migraine, based on IgG against foods: a clinical double-blind, randomised, cross-over trial,” Cephalalgia, vol. 30, no. 7, pp. 829–837, 2010. View at: Publisher Site | Google Scholar
  21. N. Crawford and B. T. Rudd, “A spectrophotofluorimetric method for the determination of serotonin (5-hydroxytryptamine) in plasma,” Clinica Chimica Acta, vol. 7, no. 1, pp. 114–121, 1962. View at: Publisher Site | Google Scholar
  22. M. Su, Y. Ran, Z. He et al., “Inhibition of toll-like receptor 4 alleviates hyperalgesia induced by acute dural inflammation in experimental migraine,” Molecular Pain, vol. 14, Article ID 174480691875461, 2018. View at: Publisher Site | Google Scholar
  23. I. A. Yilmaz, A. Ozge, M. E. Erdal, T. G. Edgünlü, S. E. Cakmak, and O. O. Yalin, “Cytokine polymorphism in patients with migraine: some suggestive clues of migraine and inflammation,” Pain Medicine, vol. 11, no. 4, pp. 492–497, 2010. View at: Publisher Site | Google Scholar
  24. G. Vidarsson, G. Dekkers, and T. Rispens, “IgG subclasses and allotypes: from structure to effector functions,” Frontiers in Immunology, vol. 5, p. 520, 2014. View at: Publisher Site | Google Scholar
  25. J. Drisko, B. Bischoff, M. Hall, and R. Mccallum, “Treating irritable bowel syndrome with a food elimination diet followed by food challenge and probiotics,” Journal of the American College of Nutrition, vol. 25, no. 6, pp. 514–522, 2006. View at: Publisher Site | Google Scholar
  26. M. H. Floch, “The role of prebiotics and probiotics in gastrointestinal disease,” Gastroenterology Clinics of North America, vol. 47, no. 1, pp. 179–191, 2018. View at: Publisher Site | Google Scholar
  27. L. Vitetta, D. Briskey, H. Alford, S. Hall, and S. Coulson, “Probiotics, prebiotics and the gastrointestinal tract in health and disease,” Inflammopharmacology, vol. 22, no. 3, pp. 135–154, 2014. View at: Publisher Site | Google Scholar
  28. M. Xuelian, Y.-K. Mao, B. Wang, J. D. Huizinga, J. Bienenstock, and W. Kunze, “Lactobacillus reuteri ingestion prevents hyperexcitability of colonic DRG neurons induced by noxious stimuli,” American Journal of Physiology-Gastrointestinal and Liver Physiology, vol. 296, no. 4, pp. G868–G875, 2009. View at: Publisher Site | Google Scholar
  29. T. N. Chase and D. L. Murphy, “Serotonin and central nervous system function,” Annual Review of Pharmacology & Toxicology, vol. 13, pp. 181–197, 2003. View at: Google Scholar
  30. J. B. Furness, “The enteric nervous system and neurogastroenterology,” Nature Reviews Gastroenterology & Hepatology, vol. 9, no. 5, pp. 286–294, 2012. View at: Publisher Site | Google Scholar

Copyright © 2019 Yangzhi Xie et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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