DNA Methylation May be Involved in the Analgesic Effect of Hyperbaric Oxygen via Regulating FUNDC1

Figure 2

Qualitative and quantitative western blot and mRNA expressive levels of DNMT1, DNMT3a, and DNMT3b after treatment with S, S + HBO, CCI, and CCI + HBO groups.(a) DNMT3a protein showed time-dependent increased expression with the highest point on the 14th day in L4 and L5 DRG, but not DNMT1 and DNMT3b (n = 6 rats per group for three repeats, presented compared with the S group, ). (b) Western blot and qt-PCR also presented that DNMT3a became gradually stronger after CCI. And the protein and mRNA expression of DNMT3a in the CCI group was stronger than that in the CCI + HBO group (n = 6 rats per group for three repeats, presented compared with the S group, ). (c) Western blot in the CCI group is stronger than that in the CCI + HBO group (presented compared with the S group, ). Qualitative and quantitative protein and mRNA expression of DNMT1 and DNMT3b after treatment in all groups had no significant difference (n = 6 rats per group for three repeats, ).

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