Research Article

Hong-Hui-Xiang Alleviates Pain Hypersensitivity in a Mouse Model of Monoarthritis

Figure 2

The time course of the effects of HHX (1, 10, and 50 μl i.m.) on pain thresholds after saline (A and C) or CFA articular injection (B and D). (a) HHX at doses of 1, 10, and 50 μl did not change the mechanical hypersensitivity after saline articular injection (time × group interaction, F(20, 200) = 1.264, ; F(20, 200) = 0.8155, ; F(20, 200) = 0.8660, ). (c) Rectangular box denoted postdrug administration behavioral assessment at different points in time after the first injection. Mechanical allogynia decreased moderately 10 min after the first injection of HHX (50 μl). (b) HHX at doses of 10 μl and 50 μl changed the mechanical hypersensitivity after CFA articular injection (time × group interaction, F(20, 200) = 7.859, ; F(20, 200) = 11.84, ). However, HHX at a dose of 1 μl had no antinociceptive effects (time × group interaction, F(20, 200) = 1.336, ). (d) The rectangular box denotes postdrug administration behavioral assessment at different points in time after the first injection. The first injection of HHX provided rapid antinociception at the hind paw at 2 h after the injection and lasted for approximately 4 h. The time of the injection of HHX (1 μl) is shown as gray arrows and HHX (10 and 50 μl) shown as black arrows. versus CFA + saline. versus baseline.