Surgical Incision Induces Anxiety-Like Behavior and Amygdala Sensitization: Effects of Morphine and Gabapentin

Figure 5

Analgesics inhibited incision-induced amygdala sensitization. (a)–(d) the increased p-CREB immunoreactivity in bilateral CeA (a) and (b) and BLA (c) and (d) in saline-injection at 1 hour following hind-paw incision. (e)–(l) Effect of morphine (e)–(h) and gabapentin (i)–(l) on p-CREB expression in the amygdala at 1 hour after incision. 10 mg/kg morphine inhibited the upregulation of p-CREB immunoreactivity in bilateral CeA (e) and (f) and BLA (g) and (h). 300 mg/kg gabapentin also inhibited the increased p-CREB expression in CeA (i) and (j) and BLA (k) and (l). (m) and (n): Quantitative analysis of the OD of p-CREB immunoreactivity in CeA (m) and BLA (n). Bar, 100 m *$P<.05$ versus the ipsilateral nucleus in saline-injection control. $n=5$ for each group.

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