Ovarian Development and Vitellogenin Gene Expression under Heat Stress in Silkworm, Bombyx mori

Figure 4

Vitellogenin mRNA expressions in pupae. (a) Fat body, day 2 pupae. (b) Ovary, day 2 pupae. (c) Fat body and ovary, day 4 pupae. The upper panel showed expression of vitellogenin and rp49 as revealed by semiquantitative RT-PCR. The lower panel showed relative expression level of vitellogenin as normalized with rp49 transcript level. Each histogram bar represents the mean relative expression of vitellogenin (–7) and the error bar represents standard error of the mean (SEM). The asterisks show the data which are significantly different from control sample (unpaired -test; , , and ). The abbreviations used are as mentioned for Figure 1.

(a)

(b)

(c)