Abstract
Purpose. The feasibility of hyperthermic isolated limb perfusion
(HILP) with tumor necrosis factor-α (TNFα ) and cisplatin for the management of
osteosarcoma was studied in the canine model.Methods. During seven perfusions in six healthy mongrel dogs
(weight 32±2 kg) technical aspects of HILP under mild hyperthermia (39– 40℃) were
studied. In five experiments HILP was performed with TNFα alone (0.5 mg/l extremity volume),
and in two experiments TNFα was combined with cisplatin (25 mg/l extremity volume).
During the perfusions physiological parameters were monitored and TNFα and total
cisplatin concentrations were determined.Results. Perfusion conditions (pH,