miRNA Profiling: How to Bypass the Current Difficulties in the Diagnosis and Treatment of Sarcomas
Figure 1
Cancer cell miRNA signatures were consistent with their tissue origin and with their sensitivity to ifosfamide. (a) This unsupervised hierarchical clustering only conserved the 61 miRNAs which discrimated osteosarcoma cells according to their response to treatment. Osteosarcoma cell lines clustered together near chondrosarcoma cells and independently to the lymphoma Daudi and Namalwa cells. Each row represents the relative levels of expression for each miRNA, and each column shows the expression levels for each sample. The red or green colour indicates relatively high or low expression, respectively, while grey squares indicate no expressed miRNA. (b) and (c) miRNA profiles after exposure to 50 μM ifosfamide for 24 h. (b) This unsupervised hierarchical clustering only conserved the 61 miRNA differently expressed in osteosarcoma cells according to their sensitivity to ifos following an ANOVA (). (c) This supervised hierarchical clustering conserved miRNAs differently expressed in cells according to their response to treatment following an ANOVA () after removing the miRNAs whose expression depends on the cell types. The red or green colour indicates relatively high or low expression, respectively, while grey squares indicate no expressed miRNA.
