miRNA Profiling: How to Bypass the Current Difficulties in the Diagnosis and Treatment of Sarcomas
Figure 2
miRNA expression profiles osteosarcoma cells were specific for each chemotherapeutic agent. (a) This unsupervised hierarchical clustering conserved the miRNAs identified as discriminating for ifosfamide response after removing the miRNAs whose expression depends on the cell types. Each row represents the relative levels of expression for each miRNA, and each column shows the expression levels for each sample. The red or green colour indicates relatively high or low expression, respectively, while grey squares indicate no expressed miRNA. (b) Cell growth was measured by the Cell Titer GloLuminescent assay as described in Section 2.6 24, 48, and 72 h after exposure to 100 μM methotrexate. Results were represented as the mean % of proliferation normalized to untreated cells of two independent experiments realized in duplicate.
