Involvement of the Soluble Urokinase Receptor in Chondrosarcoma Cell Mobilization

Table 3

SuPAR-dependent migration of chondrosarcoma cells.

Supplements	Effector	Cell migration (%)
		HT1080 cells	Chondrosarcoma cells
DMEM	DMEM	100	100
	CM LB6	110 ± 5	112 ± 8
	CM LB6/SuPAR	401 ± 12**	363 ± 16**
	10nM DI1–87	115 ± 6	105 ± 8
	10nM DII88–183	265 ± 9**	215 ± 5**
	10nM DIII184–284	98 ± 9*	100 ± 6
	10nM DIIDIII88–284	259 ± 11**	221 ± 13*
	10nM DIDIIDIII1–284	270 ± 15*	240 ± 10**
NRS	DMEM	100 ± 3	100 ± 2
	CM LB6/SuPAR	398 ± 9**	351 ± 6**
	10nM DII88–183	271 ± 5**	225 ± 11**
	10nM DIIDIII88–284	262 ± 9**	200 ± 8**
	10nM DIDIIDIII1–284	250 ± 9*	236 ± 13*
399 Anti-uPAR Ab	DMEM	100 ± 7	100 ± 5
	CM LB6/SuPAR	145 ± 12	133 ± 10
	10nM DII88–183	115 ± 6	124 ± 9
	10nM DIIDIII88–284	106 ± 11	99 ± 13
	10nM DIDIIDIII1–284	110 ± 17	102 ± 14
Anti-uPAR84–95 Ab	DMEM	100 ± 6	100 ± 5
	CM LB6/SuPAR	124 ± 9	130 ± 6
	10nM DII88–183	104 ± 13	101 ± 7
	10nM DIIDIII88–284	102 ± 9	119 ± 15
	10nM DIDIIDIII1–284	107 ± 11	110 ± 13

Cells incubated with diluents (DMEM) or 5 μg/mL the indicated antibody for 1 h at 37°C were seeded in Boyden chambers for cell migration assays as described in the Material and Method section, in the presence or absence of the indicated effectors. Conditioned medium (CM) of LB6/hSuPAR cells was used as a source of SuPAR. For quantitative analysis of cell migration, the basal value (DMEM) was taken as 100% and all values were reported relative to that. Data are the means ± SD of two independent experiments, performed in duplicate. Statistical significance with 𝑃 values was calculated against the control DMEM. *Statistical significance with 𝑃<.05. **Statistical significance with 𝑃<.001.