Figure 3: ALCAM expression in human osteosarcoma patient tumor samples. Immunohistochemistry studies in human paraffin-embedded localized and pulmonary metastatic osteosarcoma samples show mild to strong expression of ALCAM (brown) both membranous and cytoplasmic in appearance in comparison to normal human muscle and human lung. Tissues were counterstained with hematoxylin (magnification 20x).