Sarcoma / 2012 / Article / Fig 4

Research Article

Characterization of Liposarcoma Cell Lines for Preclinical and Biological Studies

Figure 4

Stem cell phenotypes of the LPS cell lines. (a) NANOG, OCT4, and SOX2 (normalized to TBP) were determined by qRT-PCR and presented relative to the average expression of each gene (log scale). (b) Cell surface protein expression of CD90, CD105, CD73, and CD44 was determined by flow cytometry. (c) Expression of CD133 and Aldefluor activity was determined by flow cytometry. (d) Colony-forming ability is presented as colonies >50  m generated from 1000 cells, following 14 days culturing in semi-solid medium.
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