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347417.fig.005b
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Figure 5: Effects of rAAV-mediated gene transfer on matrix accumulation and chondrogenic cell differentiation marker expression in transduced primary human chondrosarcoma in situ. Human chondrosarcoma explant cultures were transduced as described in Figure 4 and processed after 20 days for safranin O staining (a) and for immunodetection of type II collagen (b), type I collagen (c), type X collagen (d), SOX9 (e), and S-100 (f). All at magnification ×20 except for safranin O staining (magnification ×10).