Review Article
Discovery of Biomarkers for Osteosarcoma by Proteomics Approaches
Table 2
Summary of the platforms in proteomic analysis.
| ā | Gel-based methods | Gel free methods | Microarray | ā | 2D gel electrophoresis | 2D-DIGE | SELDI and MALDI | LC-MS | Protein array |
| Advantage | Separation of large number of proteins | Reliable quantification Required small amount of proteins Reproducible Separation of large number of proteins | Automation Required small amount of proteins Possibility of quantification | Automation Required small amount of proteins Possibility of quantification | High-throughput Automation High reproducible Required small amount of proteins Sensitive detection for post translational modification |
| Disadvantage | Required large amount of proteins Nonautomation Limitation for large and small proteins in directions | Nonautomaition Limitation for large and small proteins in directions | Less reliable in protein direction Less detection of low abundance proteins | Less detection of low abundance proteins | Limitation for the total number of proteins |
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2D-DIGE: 2-dimensional fluorescence difference gel electrophoresis, SELDI: surface-enhanced laser desorption/ionization, MALDI: matrix-assisted laser desorption/ionization, LC-MS: liquid chromatography mass spectrometry.
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