Figure 4: Gene expression in primary EWS tumors, cell lines, normal adult tissues, and hMSCs. Normalized Affymetrix gene expression data from 10 EWS cell lines (purple boxes), 32 EWS primary tumors (hatched purple boxes), 11 adult tissues (each in triplicate; green boxes), and 3 hMSC primary cultures (red boxes) were analyzed, and 29 of 36 hypermethylated genes were found to be differentially expressed among the 4 cell/tissue types (FDR < 0.05). Mean expression in each group is plotted relative to the mean expression level in all samples (error bars represent standard error of the mean). Asterisks denote the 12 genes that showed decreased expression in EWS primary tumors compared to hMSC ( ).