DNA Methylation and Gene Expression Profiling of Ewing Sarcoma Primary Tumors Reveal Genes That Are Potential Targets of Epigenetic Inactivation

Figure 5

LYN and CYP1B1 methylation analysis. (a) Schematic diagram of LYN showing the location of the Tss in relation to the CpG island, bisulfite sequencing primers, and the CpG analyzed by beadchip assay. (b) Bisulfite sequencing of LYN in EWS primary tumors and hMSCs. (c) Bead chip β scores for each sample shown. (d) qRT-PCR for LYN in EWS cell lines with or without 5-AZA treatment. Error bars demonstrating the standard error of the mean (SEM) are shown. (e) Schematic diagram of CYP1B1. (f) Bisulfite sequencing of CYP1B1 in EWS primary tumors and hMSCs. (g) Bead chip β scores for CYP1B1. (h) qRT-PCR for CYP1B1 in EWS cell lines with or without 5-AZA treatment.