Assessment of Minimal Residual Disease in Ewing Sarcoma
Figure 2
Example of how enrichment can improve the identification of cultures A673 Ewing sarcoma cells mixed with peripheral blood mononuclear cells: (a) No tumor cells are identified in a healthy volunteer blood sample analysis not containing tumor cells (negative control). (b) Conventional flow cytometry without enrichment identifies equivocal findings of a single event (red dot) in a sample containing one A673 Ewing sarcoma cell per 1 × 106 pbmc. (c) In contrast, use of enrichment technique allows for confident identification of tumor cells (e.g., cluster of 5 or more events) that are CD99+/CD45- in a sample containing one A673 cell per 1 × 106 pbmc. (d) Positive control containing only A673 tumor cells.
