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Sarcoma
Volume 2016, Article ID 1390571, 11 pages
http://dx.doi.org/10.1155/2016/1390571
Research Article

miR-125b and miR-100 Are Predictive Biomarkers of Response to Induction Chemotherapy in Osteosarcoma

1Division of Rare Cancer Research, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
2Division of Musculoskeletal Oncology, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
3Division of Molecular and Cellular Medicine, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
4Pathology and Clinical Laboratory Division, National Cancer Center Hospital, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
5Division of Cancer Genomics, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan
6Department of Functional Analysis, National Cancer Center Research Institute, 5-1-1 Tsukiji, Chuo-ku, Tokyo 104-0045, Japan

Received 11 July 2016; Revised 4 October 2016; Accepted 18 October 2016

Academic Editor: Valerae O. Lewis

Copyright © 2016 Daisuke Kubota et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

List of supplementary materials

Supplementary List

Supplementary Figure 1

Effects of expression of miR-100 and miR-125b on drug sensitivity

Supplementary Figure 2

Effects of miR-100 and miR-125b on apoptosis

Supplementary Table 1

Expression data of six miRNAs by microarray

Supplementary Table 2

Expression data of six miRNAs by qRT-PCR

Supplementary Table 3

miRNAs changed sensitivity to chemotherapy drugs

Supplementary Table 4

Genes the expression of which was affected by miRNA-100 transfection

Supplementary Table 5

Genes the expression of which was affected by miRNA-125b transfection

Legends of supplementary figures

Supplementary Figure 1

Effects of expression of miR-100 and miR-125b on drug sensitivity. Expression of miR-100 and miR-125b was silenced in MNNG/HOS (A, B, C), 123B (D, E, F), and MG63 (G, H, I) cells, which were then exposed to methotrexate (A, D, G), doxorubicin (B, E, H), and cisplatin (C, F, I). IC50 values are summarized in Supplementary Table 3.

Supplementary Figure 2

Effects of miR-100 and miR-125b on apoptosis. Expression of apoptosis proteins was measured before and after transfection with miRNA-125b (A). Over-expression of miRNA-125b decreased expression of Bak1, p53, Caspase-2, MCL-1, PUMA, and PP2A catalytic α (A). miRNA-125b partially blocked the ability of methotrexate (MTX), doxorubicin (DOX), and cisplatin (CDDP) to suppress expression of Bak1 (B, upper panel), and induce cleaved PARP (B, middle panel). On the other hand, miR-100 inhibited the Rb pathway (C).

  1. Supplementary Material