Axitinib Has Antiangiogenic and Antitumorigenic Activity in Myxoid Liposarcoma

Figure 2

FUS-CHOP and eIF4E are critical for myxoid liposarcoma survival and promote the angiogenic properties of myxoid liposarcoma. MLS 1765 cells were treated with CHOP-directed (a), eIF4E-directed (b), or scramble siRNA and the percentage of protein reduction was determined by densitometry. MLS 402 and MLS 1765 cells were treated with CHOP-directed, eIF4E-directed, or scramble siRNA for 5 days and cell viability determined by MTS uptake (c). Three independent experiments were performed, and the data are presented as the mean + SEM. MLS 1765 cells treated with CHOP-directed, eIF4E-directed, or scramble siRNA for 5 days, and then RT-qPCR was performed to quantify the expression of VEGF ligands and receptors. Data are presented relative to the housekeeping gene GAPDH; three independent experiments were performed; and the data are presented as the mean + SEM (d). HUVECs were suspended in Matrigel, and then conditioned medium from MLS 402 cells pretreated with CHOP-directed, eIF4E-directed, or scramble siRNA was applied to the HUVECs. Images were acquired at hourly intervals, and the figure displays representative images taken at 8 h (left). Tube lengths were measured using ImageJ (version 1.47d). Three independent experiments were performed, and the data are presented as the mean + SEM (e). CM, conditioned medium; IB, immunoblotting; LO, Lipofectamine-only treated cells; Ut, untreated MLS cells. A paired, two-tailed -test was performed. ; ; ; .

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